KMID : 1007520200290111511
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Food Science and Biotechnology 2020 Volume.29 No. 11 p.1511 ~ p.1518
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Sea buckthorn (Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells
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Zhang Ting
Qin Xuze Cao Yuxin Zhang Jianxin Zhao Junxing
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Abstract
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The objective of this study is to investigate the effects of sea buckthorn oil (SBO) on proliferation, adipogenic differentiation and insulin sensitivity of 3T3-L1 cells. Results showed that SBO increased cell proliferation ability, accompanied by up-regulated proliferating cell nuclear antigen content (p?0.05) and p38 activity (p?0.05). SBO also promoted adipogenesis and enhanced adipogenic transcriptional factors expression. Mitochondrial biogenesis related gene expressions were elevated in SBO treated cells (p?0.05). Of note, SBO also increased glucose uptake and glucose transporter 4 abundance (p?0.05). Cells treated with SBO exhibited greater phosphorylated insulin receptor substrate 1 (p?0.05), phosphorylated-Akt (p?0.05) and phosphorylated AMP-activated protein kinase (p?0.01) contents. When taken together, these results suggest that SBO promotes 3T3-L1 cells proliferation, adipogenesis and insulin sensitivity.
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KEYWORD
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Sea buckthorn oil, Proliferation, Adipogenesis, Insulin sensitivity, AMP-activated protein kinase
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